抗体GAPDH Monoclonal antibody

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, ROS1728 cells, pig brain tissue, zebrafish tissue, whole yeast, whole Nematode tissue, soybean whole plant tissue, arabidopsis whole plant tissue, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, C6 cells, PC-12 cells, C2C12 cells, SP2/0 cells, rat brain tissue, mouse brain tissue

Positive IP detected inHeLa Cells

Positive IF detected inEthacrynic acid treated HeLa cells

Positive FC detected inHeLa cells

Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.

推荐稀释比

APPLICATIONDILUTION

Western Blot (WB)WB : 1:50000-1:500000

Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

Immunofluorescence (IF)IF : 1:200-1:2000

Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

发表文章中的应用

KD/KOSee 1 publications below

WBSee 9065 publications below

IHCSee 9 publications below

IFSee 23 publications below

IPSee 28 publications below

FCSee 3 publications below

CoIPSee 11 publications below

产品信息

60004-1-Ig targets GAPDH in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat, yeast, plant, zebrafish, pig samples.

Tested ApplicationsFC, IF, IP, WB, ELISA

Cited ApplicationsCell Treatment, CoIP, FC, IF, IHC, IP, WB

Tested ReactivityHuman, Mouse, Rat, Yeast, Plant, Zebrafish, Pig

Cited ReactivityHuman, Rat, Deer, Goat, Tick, Treeshrew, Cynomorium Songaricum, Chicken, Whiteflies, Yeast

ImmunogenGAPDH fusion protein Ag0766 种属同源性预测

Host / IsotypeMouse / IgG2b

ClassMonoclonal

TypeAntibody

Full Nameglyceraldehyde-3-phosphate dehydrogenase

SynonymsG3PD, GAPD, GAPDH, OK/SW cl.12

Calculated molecular weight36 kDa

Observed molecular weight36 kDa

GenBank accession numberBC004109

Gene symbolGAPDH

UniprotP04406

Gene ID (NCBI)2597

RRIDAB_2107436

ConjugateUnconjugated

FormLiquid

Purification MethodProtein A purification

Storage BufferPBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the molecular weight of GAPDH?

The molecular weight of GAPDH is 36 kD.

What is GAPDH?

GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" 

protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic 

production of ATP and pyruvate (PMID: 21895736).

What is the function of GAPDH?

GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even 

critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH 

is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting 

as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in 

transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 

11035021).

Where in the cell is GAPDH localized?

GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus 

(PMIDs: 21895736, 12829261).

Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear 

functions?

Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is 

useful as a control for Western blots and RT-PCR.

What are the known protein-protein interactions of GAPDH?

GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of 

these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).

Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.