经过测试的应用
Positive WB detected in human placenta tissue, HepG2 cells, HEK-293 cells, HeLa cells, Raji cells, K-562 cells, mouse heart tissue, PC-13 cells, arabidopsis whole plant tissue, corn whole plant tissue, mouse brain tissue, mouse skin tissue, Jurkat cells, NIH/3T3 cells, C6 cells, rat brain tissue, RAW 264.7 cells
Positive IP detected in A549 cells
Positive IHC detected in human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in HeLa cells, HepG2 cells
Positive FC detected in HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.
推荐稀释比
APPLICATION DILUTION
Western Blot (WB) WB : 1:5000-1:40000
Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC) IHC : 1:200-1:800
Immunofluorescence (IF) IF : 1:500-1:2000
Flow Cytometry (FC) FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery
发表文章中的应用
WB See 5425 publications below
IHC See 7 publications below
IF See 8 publications below
IP See 13 publications below
ELISA See 2 publications below
CoIP See 10 publications below
RIP See 2 publications below
产品信息
10494-1-AP targets GAPDH in WB, RIP, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA applications and shows reactivity with human, mouse, rat, pig, arabidopsis , corn , cabbage, rice samples.
Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications Cell Treatment, CoIP, ELISA, IF, IHC, IP, RIP, WB
Tested Reactivity Human, Mouse, Rat, Pig, Arabidopsis , Corn , Cabbage, Rice
Cited Reactivity Human, Rat, Goat, Treeshrew, Chicken, Yeast, Leech, Arabidopsis, Zebra Finches, Sheep
Immunogen GAPDH fusion protein Ag0766 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glyceraldehyde-3-phosphate dehydrogenase
Synonyms G3PD, GAPD, GAPDH, OK/SW cl.12
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession number BC004109
Gene symbol GAPDH
Uniprot P04406
Gene ID (NCBI) 2597
RRID AB_2263076
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.
背景介绍
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
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