抗体DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)

原价

￥980.00-6000.00

售价

￥ 882.00-5400.00

0销量

可选规格

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经过测试的应用

Positive WB detected in Transfected HEK-293 cells

Positive IP detected in Transfected HEK-293 cells

Positive IF detected in Transfected HEK-293 cells

推荐稀释比

APPLICATION DILUTION

Western Blot (WB) WB : 1:5000-1:50000

Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

Immunofluorescence (IF) IF : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

发表文章中的应用

WB See 99 publications below

IHC See 1 publications below

IF See 15 publications below

IP See 32 publications below

CoIP See 23 publications below

ChIP See 3 publications below

RIP See 1 publications below

产品信息

66008-4-Ig targets DYKDDDDK tag in WB, RIP, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications IF, IP, WB, ELISA

Cited Applications ChIP, CoIP, IF, IHC, IP, RIP, WB

Tested Reactivity Recombinant Protein

Cited Reactivity Human, Mouse, Chicken, Zebrafish, Pig, Canine, Bovine

Immunogen Peptide 种属同源性预测

Host / Isotype Mouse / IgG2b

Class Monoclonal

Type Antibody

Full Name DYKDDDDK tag

Synonyms DDDDK, DDDDK tag, DYKDDDDK, DYKDDDDK tag, Flag, Flag tag

Gene symbol

Gene ID (NCBI)

RRID AB_2918475

Conjugate Unconjugated

Form Liquid

Purification Method Protein A purification

Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage Conditions Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

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￥882.00-5400.00

抗体Beta Actin Monoclonal antibody

生物试剂

抗体DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope)