抗体HA tag Polyclonal antibody

经过测试的应用

Positive WB detected in recombinant protein, Transfected HEK-293 cells

Positive IP detected in Transfected HEK-293 cells

Positive IF detected in Transfected HEK-293 cells

推荐稀释比

APPLICATION DILUTION

Western Blot (WB) WB : 1:5000-1:10000

Immunoprecipitation (IP) IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

Immunofluorescence (IF) IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

发表文章中的应用

WB See 442 publications below

IHC See 3 publications below

IF See 39 publications below

IP See 131 publications below

FC See 1 publications below

CoIP See 33 publications below

ChIP See 6 publications below

产品信息

51064-2-AP targets HA tag in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications IF, IP, WB, ELISA

Cited Applications ChIP, CoIP, FC, IF, IHC, IP, WB

Tested Reactivity Recombinant Protein

Cited Reactivity Human, Mouse, Rat, Chicken, Monkey, Zebrafish, Duck, Canine

Immunogen Peptide 种属同源性预测

Host / Isotype Rabbit / IgG

Class Polyclonal

Type Antibody

Full Name HA tag

Synonyms HA tag, YPYDVPDYA

Calculated molecular weight 1 kDa

Gene symbol

Gene ID (NCBI)

RRID AB_11042321

Conjugate Unconjugated

Form Liquid

Purification Method Antigen affinity purification

Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage Conditions Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.